It is also available in Spain with Bifidobacterium longum. Nutr Clin Pract. 2010;83:9–13. LDL-CHO levels in both the HCD-KID7 and HCD-L.ac group were decreased by an average of 35.5 and 38.7%, respectively, compared with the HCD control group (both, P < 0.05) (Figure 4B). Int. J. Appl. A combination of calcium phosphate and probiotics beneficially influences intestinal lactobacilli and cholesterol metabolism in humans. The study was aimed to characterize the probiotic properties of a Pediococcus pentosaceus strain, KID7, by in vitro and in vivo studies. “Pediococcus pentosaceus are categorized as a “lactic acid bacteria” because the end product of its metabolism is lactic acid [5]. Immunol. Pediococcus is a Firmicute, which is the phylum that CFS/IBS/FM patients are too high in. Fuentes, M. C., Lajo, T., Carrión, J. M., and Cuñé, J. The cholesterol concentration in spent medium was estimated by enzymatic method using the BCS total cholesterol kit (Bioclinical System, Seoul, South Korea) following the manufacturer's protocol and compared with uninoculated MRS-CHO broth as control and the percentage cholesterol removal was calculated. We examined the expression levels of cholesterol metabolism-related genes to understand the mechanism of action of KID7 compared to L. acidophilus ATCC 43121 in improving hypercholesterolemia (Figure 6). Its properties are mainly attributed to the acidic composition, to the capacity of glucuronic acid to bind to toxin molecules, and to the content of B-complex vitamins and phenols. Aggregation and adhesion properties of 22 Lactobacillus strains. doi: 10.3168/jds.S0022-0302(05)72662-X, Liong, M. T., and Shah, N. P. (2005b). P. pentosaceus has been approved as an animal feed additive in the United States, European Union, China, Thailand, Australia, and New Zealand (Lim and Tan, 2009). (2014) with minor modification. Biotechnol. doi: 10.3920/BM2013.0084, Bove, P., Gallone, A., Russo, P., Capozzi, V., Albenzio, M., Spano, G., et al. 2012;26:31–42. MRS reaction mixture was prepared with 5 mM taurodeoxycholic acid (TDCA), or 5 mM glycocholic acid (GCA), or 5 mM taurocholic acid (TCA) in sodium phosphate buffer (pH 7.4). J Agric Food Chem. eCollection 2020. doi: 10.1263/jbb.106.69, Joyce, S. A., Macsharry, J., Casey, P. G., Kinsella, M., Murphy, E. F., Shanahan, F., et al. 4). Clin. Potential probiotic lactic acid bacteria Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019) do not degrade gastric mucin in vitro. The ready-to-cook minced meat with 10% tomato powder fermented with L. sakei and P. pentosaceus had the highest acceptability scores (9.4 and 8.8, respectively) (Fig. The entire assay was performed in triplicates to check the reproducibility. Environ. J. Nutr. casei, Lactobacillus casei ssp. 2013;33:1–10. Evaluation of certain food additives: 67th report of the Joint FAO/WHO Expert Committee on Food Additives. Br. Minced meat samples were covered with plastic film and stored at 4 °C for 24 h until analysis. The colour characteristics of samples were evaluated at three different positions of the surface using CIELab system (CromaMeter CR-400, Konica Minolta, Kyoto, Japan). The lycopene and β-carotene contents of the ready-to-cook minced meat increased from 0.48 to 10.32 mg per 100 g and from 0.19 to 1.95 mg per 100 g, respectively, depending on the percentage of tomato powder in the formulation (10 or 30%) (Table 2). J. Clin. Then, they are kept in a warm and dry place for 15–22 days. In our previous study (19), L. sakei and P. pentosaceus strains during tomato pulp fermentation reduced the pH up to 3.5–3.7, and the viable cell counts of the tested strains reached 6.61 log CFU/g after 48 h of tomato pulp fermentation. Chem. (2011). One milliliter of 18-h culture was mixed with an equal volume of phosphate-buffered MRS + conjugated bile acid and incubated for 36 h at 37°C. The lower growth of the KID7 and P. pentosaceus KACC 12311 in bile-supplied medium could be due to bile salt-deconjugating activity of the strains, which leads to accumulation of free bile acids that are more toxic than conjugated bile (Pereira et al., 2003). The mice were housed under temperature (23 ± 3°C) and humidity-controlled conditions (40 ± 6%) with a 12-h light/dark cycle and were given free access to water and food throughout the experiment. Meat Sci. Effects of KID7 and L. acidophilus on the serum biochemical parameters. One hundred microliter of concentrated crude culture filtrate was loaded on to an 8-mm paper disc (ADVANTEC, Japan) and dried. We proposed that these bacteria can be used widely in the food industry as biopreservatives due to their broad inhibition spectrum. In addition, spontaneous fermentation increased the content of total carotenoids, but not as effectively; the content of carotenoids in the spontaneously fermented samples was 43.7% lower compared with the lactofermented samples (Fig. Effect of the addition of tomato paste on the nutritional and sensory properties of mortadella. NCD: normal diet; HCD: high-cholesterol diet; HCD-KID7: HCD + P. pentosaceus KID7; HCD-L.ac: HCD + L. acidophilus. The lowest score was of the ready-to-cook minced meat samples with 10% nonfermented tomato powder. Lipidol. When favourable conditions of anaerobiosis, water activity, salt concentration and temperature occur, raw vegetables and fruits may be subjected to spontaneous lactic acid fermentation. The aminoglycoside resistance gene aac(6′)Ie-aph(2″)Ia has been reported in some Pediococcus species (Ammor et al., 2007), which codes for the bifunctional aminoglycoside-modifying enzyme 69-N-aminoglycoside acetyltransferase-20-O-aminoglycoside phosphotransferase (Tenorio et al., 2001). The body weight was measured once per week for 6 weeks. Analysis of expression of genes associated with lipid metabolism in liver showed that low-density lipoprotein receptor (LDLR), cholesterol-7α-hydroxylase (CYP7A1) and apolipoprotein E (APOE) mRNA expression was significantly increase in the HCD-KID7 group compared to the HCD group. 39, 824–825. J. Func. Thus, bacterial BSH activity in the gut acts as a sink for cholesterol (Joyce et al., 2014). Pediococcus pentosaceus MZF16 has been isolated from artisanal Tunisian meat so called “Dried Ossban,” an original ecological niche, and identified by MALDI-TOF mass spectrometry and 16S rDNA sequencing. According to the Genomes Online Database (http://wit.integratedgenomics.com/GOLD/), there are 717 prokaryotic and eukaryotic genome-sequencing projects ongoing or completed at the time of writing (June 2003). The effect of industrial food processing on potentially health beneficial tomato antioxidants. We also observed a significant increase in the level of hepatic LDL mRNA expression in the HCD-L.ac group, which is consistent with Park et al.